SpectraMax 190 Microplate Reader
Date：2020/3/19
The SpectraMax 190 Microplate Reader from Molecular Devices is ideal for UV-Vis life science applications, especially DNA analysis. The multi-channel design truly mimics a dual-beam spectrophotometer.
Each sample has a discrete sample beam and reference beam so that each well is measured directly, eliminating error due to variations in light output between the optic fibers. The system consists of eight sample beams and detectors and eight reference beams and detectors to deliver both
superior precision and speed-of-reading across the microplate through 4.0 OD.
Optional tools for verifying the SpectraMax 190 reader performance include the SpectraTest ABS1 Absorbance Plate and IQ/OQ/PQ manual. Software tools for regulatory compliance, such as FDA 21 CFR Part 11, are available through SoftMax Pro GxP Software.
Details
Accurate and precise absorbance readings
The quartz fiber optic system minimizes stray light for precise readings across the microplate. Each channel has its own reference detector and the small beam size maintains high performance even with small sample volumes read in half-area wells. The 2 nm bandwidth provides the needed spectral resolution to ensure accuracy of DNA absorbance measurements. Up to 6 different wavelengths can be measured during one read.
PathCheck sensor eases the workload
For aqueous solutions, the patented PathCheck Sensor senses the depth of the liquid in each microplate well and normalizes the absorbance value to a 1 cm pathlength. The corrected absorbance is within 5% of the value obtained in a conventional spectrophotometer.
The PathCheck sensor makes it is easy to:
? Use the extinction coefficient of a sample to calculate concentration directly
? Correct for volume differences in the wells
? Expand the assay’s dynamic range
? Check performance of pipetting devices
Spectral scanning for easy assay development
The complete spectrum of samples can be scanned to select the optimum wavelength for reading. Spectrum scanning also allows:
? Monitoring of sample or reagent stability
? Determination of interfering compounds or assay conditions
Performs virtually any UV/VIS assay
? Bacterial identification
? Entomology
? Biochemistry
? Environmental testing
? Cell biology
? Enzyme kinetics
? Colorimetric
? Food/agricultural
? DNA quantitation
? Molecular biology
? ELISAs
? Pharmacology
Accurate measurements
The SpectraMax 190 reader is the tool for DNA analysis. The ability to detect 16 ng/well of DNA and to quantitate 50 ng of DNA provides the same sensitivity as a spectrophotometer. The PathCheck sensor can extend the instrument linearity by a factor of three or more. For very concentrated samples, use a minimum volume in the microplate well (100 μL has a pathlength of about 0.30 cm), then use the PathCheck sensor to calculate an equivalent 1-cm pathlength absorbance value.
Ensure GxP compliance
The SpectraTest validation package provides a NIST-traceable solution for validating the performance of the SpectraMax 190 reader automatically.
Plate stacker and robot integration
The SpectraMax 190 reader can be integrated with Molecular Devices’ StakMax? Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The “out-of-the-box” automation solution saves up-front integration time and resources.
